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Video

Otology/Neurotology

(A827) Heterogeneity and Microenvironment in Temporal Bone Squamous Cell Carcinoma Based on Single-cell Ribonucleic Acid Sequencing

Monday, October 13, 2025
12:00 PM - 1:00 PM EDT
Location: OTO EXPO, Posters

    Presenting Author(s)

  • Wen Lu, MD

    Physician
    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai, Shanghai, China (People's Republic)

    • Co-Author(s)

  • Yi-Ni Li, MD

    Resident
    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai, Shanghai, China (People's Republic)

  • JL

    Jie Li, MD

    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, United States

  • Tianjiao zhou

    Dr
    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai, Shanghai, China (People's Republic)

  • DY

    Dongzhen Yu, PhD

    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, United States

  • HS

    Hai-Bo Shi, PhD

    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China (People's Republic)

    • Senior Author(s)

  • HS

    Hai-Bo Shi, PhD

    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China (People's Republic)

Disclosure(s):
Wen Lu, MD: No relevant relationships to disclose.
Yi-Ni Li, MD: No relevant relationships to disclose.


Introduction: Temporal bone squamous cell carcinoma (TBSCC) is the most common malignant tumor of the temporal bone, yet its rarity and frequent misdiagnosis obscure its pathological mechanisms and treatment approaches. This study aimed to employ single-cell RNA sequencing to dissect tumor heterogeneity and the microenvironment of TBSCC, providing new insights into its diagnosis, classification, and treatment.

Methods: Single-cell RNA sequencing analyzed five cancer and four paracancerous tissue samples to map the single-cell transcriptome, focusing on cell composition, cell cycle, pseudo-time trajectories, and immune cell variation.

Results: Results revealed significant inter-patient heterogeneity in cell composition, cell cycle, and pseudo-time trajectories, with varying immune cell and T cell subtype compositions across samples. Systematic comparisons identified distinct expression patterns of differentially expressed genes linked to tumorigenesis and tumor subtype formation.

Conclusions: This study is the first to unveil the complex heterogeneity of TBSCC, including tumor microenvironment characteristics and differentially expressed genes, offering new avenues for diagnosing, classifying, and treating this carcinoma.