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Video

Laryngology/Broncho-Esophagology

(A718) Histological Investigation and Comparative Analysis of a Novel 455nm Blue Light Laser

Monday, October 13, 2025
12:00 PM - 1:00 PM EDT
Location: OTO EXPO, Posters

    Presenting Author(s)

  • MS

    Maxwell Scher, MD

    Resident
    Boston University Medical Center
    Boston, Massachusetts, United States

    • Co-Author(s)

  • MS

    Maxwell Scher, MD

    Resident
    Boston University Medical Center
    Boston, Massachusetts, United States

    • Presenting Author(s)

  • MM

    Margaret Mills, MD

    Resident
    University of Connecticut
    Farmington, Connecticut, United States

    • Co-Author(s)

  • MM

    Margaret Mills, MD

    Resident
    University of Connecticut
    Farmington, Connecticut, United States

    • Author(s)

  • Denis Lafreniere, MD

    Assoc. Dean of Clinical Affairs Medical Director of UMG Professor & Chief Div. of Otolaryngology
    University of Connecticut
    Farmington, Connecticut, United States

  • GG

    Gregory A. Grillone, MD

    Strong-Vaughan Professor & Chair | Chief Department of Otolaryngology-Head &Neck Surgery
    Boston University Medical Center
    Boston, Massachusetts, United States

    • Senior Author(s)

  • Lauren F. Tracy, MD

    Assistant Professor, Otolaryngology
    Boston Medical Center
    Boston, Massachusetts, United States

Disclosure(s):
Maxwell Scher, MD: No relevant relationships to disclose.
Margaret Mills, MD: No relevant relationships to disclose.
Denis Lafreniere, MD: No relevant relationships to disclose.


Introduction: To histologically evaluate cutting tissue and angiolytic effects of a novel 455nm laser over a range of settings and compare with a 10,600nm fiber-based carbon dioxide (CO2) laser.

Methods: Using the 455nm blue laser, 300µm and 500µm fibers were secured at specific distances (1-5mm) from ex-vivo bovine tongue tissue. The tissue was moved at a fixed speed of 2mm/second and used to incise ventral tongue tissue at 3 distinct locations at variable power (5-20W). The same was performed with a 10,600nm laser with 300µm and 500µm fibers. Histologic specimens were stained for hematoxylin and eosin and lactate dehydrogenase. Measurements including incision depth and spread of thermal damage were compared between different wavelengths, power, and distances from the tissue.

Results: Average incision depth at 5W for the 455nm laser and 10,6000nm laser was 0.448mm (SD:0.19mm) vs 0.643mm (SD:0.25mm) respectively (p=0.087). Average incision depth at 10W for the 455nm laser and 10,6000nm laser was 1.007mm (SD:0.33mm) vs 1.309mm (SD:0.49mm) respectively (p=0.090). Increased power on the 455nm laser resulted in increased incision depth (5W: 0.448mm vs 20W: 1.588mm, p< 0.0001). Distance from tissue did not significantly impact incision depth. Thermal damage depth of the 455nm laser averaged 0.469mm vs 0.149mm for the 10,600nm laser (p < 0.0001). Thermal damage depth did not change significantly with power for the 455nm or 10,6000nm laser.

Conclusions: In ex-vivo tongue, the novel 455nm laser creates similar incision depth, but greater thermal damage depth in comparison to the 10,600nm laser at similar power. Increased power for the 455nm laser resulted in increased incision depth but not thermal damage spread.