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Video

Otology/Neurotology

(A859) Redistribution of Oligodendrocyte Phenotypes in the Medial Vestibular Nuclei After Unilateral Labyrinthectomy

Monday, October 13, 2025
12:00 PM - 1:00 PM EDT
Location: OTO EXPO, Posters

    Presenting Author(s)

  • JL

    Jie Li, MD

    resident
    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai, China (People's Republic)

    • Co-Author(s)

  • Yi-Ni Li, MD

    Resident
    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai, Shanghai, China (People's Republic)

  • Wen Lu, MD

    Physician
    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
    Shanghai, Shanghai, China (People's Republic)

  • ZS

    Zheng-Yu Shi, MS

    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, United States

    • Senior Author(s)

  • HS

    Hai-Bo Shi, PhD

    Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China (People's Republic)

Disclosure(s):
Jie Li, MD: No relevant relationships to disclose.
Yi-Ni Li, MD: No relevant relationships to disclose.
Wen Lu, MD: No relevant relationships to disclose.


Introduction: Oligodendrocytes cells are highly heterogeneous and commonly involved in the regulation of neural circuit function. However, how the oligodendrocytes in the deafferented vestibular nuclei reacts to the acute unilateral vestibular dysfunction remains unclear. Our study aims to investigate the response status of oligodendrocytes in the medial vestibular nucleus (MVN) after unilateral vestibular loss.

Methods: The medial vestibular nucleus was harvested from C57BL/6J mice that were sacrificed 1 day after unilateral labyrinthectomy and then dissociated into single-cell suspension. The cDNA library was constructed and sequenced on Illumina HiSeqX10. Downstream analysis was performed using the R packages.

Results: After unilateral labyrinthectomy (UL), we observed the distribution of oligodendrocyte lineage cells altered. Oligodendrocyte precursor cells increased after vestibular loss. A cluster of highly expressed Tcf7l2 and Enpp6 was defined as premyelinating oligodendrocytes, which is one of the dominant oligodendrocyte subtypes in the deafferented MVN but rarely seen in the control group. We observed an elevated expression level of Fyn in the UL group on the first day after surgery. The number of EDU-labeled Pdgfra (+) OPCs and CC1 (+) matured oligodendrocytes increased in the UL group seven days post-surgery, indicating that OPCs proliferation and differentiation were promoted.

Conclusions: Our findings reveal the adaptive changes of oligodendrocyte subtypes in the early vestibular compensation following peripheral vestibular damage, implicating the potential roles of oligodendrocytes in remodeling neural circuits to reverse behavioral deficits.